1
کشاورزی::
طیف سنجی جرمی
In this chapter, we assume that the reader understands the fundamentals of gas chroma- tography and mass spectrometry.
Two-dimensional gas chromatography (GC GC) with time-of-flight mass spectrometry (TOF-MS) continues to increase in popularity (Nicolotti et al.
3.2 Gas chromatography and mass spectrometry
Electron impact is the usual ionisation mode used for aroma compound identifica- tion by mass spectrometry.
In the past, compounds with weak or non-existent molecular ions, such as aldehydes or alco- hols, may have been identified using chemical ionisation mass spectrometry (Harrison, 1992), where fragmentation is caused by a charged reagent gas (e.g. ammo- nia, isobutane or methane) rather than electrons, giving an obvious protonated molec- ular ion (M + 1).
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2
عمومی::
طیفسنجی جرمی
Mass spectrometry (MS) has both high sensitivity and high specificity.
Applications of MS for the detection of antimicrobial resistance in bacteria generally require growing pure cultures first to simplify the MS profile.
MS methods are based on the measurement of a discrete and reproducible set of molecular biomarkers in these cultures that may be correlated with specific attributes.
Identification of bacteria based on the detection of specific chemical species by MS has been referred to as MS‐based chemotaxonomy.
Some of the MS methods that have been developed thus far allow the rapid determination of antibiotic resistance in previously identified bacteria, but not in real unknowns.
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